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Literature summary extracted from

  • Coey, C.T.; Drohat, A.C.
    Defining the impact of sumoylation on substrate binding and catalysis by thymine DNA glycosylase (2018), Nucleic Acids Res., 46, 5159-5170 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.29 expressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.29 additional information sumoylation dramatically impairs the enzyme's base-excision activity, such that guanine-thymine activity is reduced by more than 45fold and 5-formylcytosine and 5-carboxylcytosine are excised slowly, with a reaction half-life of more than 9 min at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.29 5-carboxylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-formylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.29 Homo sapiens Q13569
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.29 Mono Q column chromatography as final chromatographic step Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.29 5-carboxylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 5-formylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
3.2.2.29 thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.29 TDG
-
 Homo sapiens
3.2.2.29 thymine DNA glycosylase
-
 Homo sapiens